http://tainguyenso.vnu.edu.vn/jspui/handle/123456789/9478 2026-05-17T13:16:19Z 2026-05-17T13:16:19Z Ngo, Van Thanh et al. http://tainguyenso.vnu.edu.vn/jspui/handle/123456789/9982 2011-05-31T02:50:31Z 2010-01-01T00:00:00Z

Vector construction and transformation of 4CL1 gene into Chinaberrytree (Melia azedarach L.) Ngo, Van Thanh; et al. The gene 4CL1 was isolated from Chinese red pine (Pinus massoniana Lamb) and ligated into vector pPTN289 to perform transformation vector pPTN289-4CL1. This construction was transformed into Agrobacterium tumefaciens strain C58, and then transformed into Chinaberrytree (Melia azedarach L.). The transgenic Chinaberrytree was screened on selection medium (MS + 0.5mg/l 6-BA + 1mg/l vitamine B5 + 30g/l sucrose + 8g/l agar + 500mg/l Cefotaxime + 1mg/l PPT) and then extracted total DNA and tested the existence of interested gene using PCR method. The result shows that two tested Chinaberrytree samples are positive with 4CL1 gene as same as the positive control, while the nagative control (wild Chinaberrytree) is nagative with 4CL1 gene.

2010-01-01T00:00:00Z Nguyễn, Thị Kim Thường Trần, Chương Huyến http://tainguyenso.vnu.edu.vn/jspui/handle/123456789/9979 2011-05-31T02:46:25Z 2010-01-01T00:00:00Z

Xác định amlodipin besylate bằng phương pháp Von-ampe hòa tan hấp phụ xung vi phân Nguyễn, Thị Kim Thường; Trần, Chương Huyến Tính chất hấp phụ và điện hóa của amlodipin besylate trên điện cực than gương đã được nghiên cứu trong môi trường đệm Britton-Robinson bằng phương pháp von-ampe vòng và phương pháp von-ampe hòa tan xung vi phân. Phương pháp von-ampe vòng cho thấy, sự oxy hóa hai electron của amlodipin besylate trên bề mặt điện cực than gương là bất thuận nghịch và có hấp phụ xảy ra trên điện cực. Các điều kiện đo của dung dịch và các thông số máy đã được tối ưu để xây dựng quy trình xác định thuốc bằng phương pháp von-ampe hòa tan hấp phụ xung vi phân. Amlodipin besylate cho một peak oxy hóa tại 0,698V (So với Ag/AgCl) tại pH = 5. Peak oxy hóa được sử dụng để xác định amlodipin besylate trong khoảng nồng độ 10-8M đến 10-6M với giới hạn phát hiện là 7,6.10-9M. Quy trình được áp dụng thành công để xác định amlodipin besylate trong một số mẫu thuốc.

2010-01-01T00:00:00Z Nguyen, Trung Thanh Paek, Kee Yoeup http://tainguyenso.vnu.edu.vn/jspui/handle/123456789/9976 2011-05-31T02:41:54Z 2010-01-01T00:00:00Z

Cell suspension culture Panax ginseng C. A. Meyer: Role of plant growth regulators and medium composition on biomass and ginsenoside production Nguyen, Trung Thanh; Paek, Kee Yoeup We established cell suspension culture on ginseng and some attempts have been made to increase ginsenoside yield of ginseng cell culture through manipulation various culture factors and process variable. The maximum biomass yields of cell suspension culture of ginseng was obtained in medium containing 2,4-D as compared to IBA or NAA. However, ginsenoside production was much higher in IBA or NAA containing medium and 7 mg/L IBA was determined to be optimal for cell growth (10.1 mg/L DW) and ginsenoside production (7.2 mg/g DW). Addition of cytokinin (BA and kinetin) did not affect cell growth but ginsenoside production was increased when the medium supplemented with 0.5 mg/L BA or 0.5 mg/L kinetin. Half and full strength MS medium were found to be equally suitable for both biomass as well as ginsenoside production. At various sucrose concentration investigated, 30 g/L sucrose enhanced biomass yield as well as ginsenoside production and further increase of sucrose concentration upto 70 g/L led to a decrease in ginsenoside accumulation and biomass production. The maximum growth and ginsenoside production was obtained for nitrogen concentration of 30 mM

2010-01-01T00:00:00Z Tran, Thi Nhu Mai et al. http://tainguyenso.vnu.edu.vn/jspui/handle/123456789/9974 2011-05-31T02:37:10Z 2010-01-01T00:00:00Z

Study on the characteristics and catalytic properties of Pt/SBA-15 in the selective oxidation of D-Glucose Tran, Thi Nhu Mai; et al. In this work, platinum nanoparticles were dispersed on SBA-15 mesoporous material by incipient wetness method and the synthesized materials were characterized by XRD, TEM, EDX spectroscopies and N2 adsorption-desorption isotherm measurement. The results indicated that 2D hexagonal ordered structure of SBA-15 was still maintained after grafting Pt on SBA-15 support and platinum nanoparticles existed both inside and outside the pore channels of SBA-15 material. Catalytic activity of these materials was tested in the aqueous phase D-glucose oxidation as a model reaction. The reaction was carried out in a glass reactor at atmospheric pressure, 80oC, air flow rate 20ml/minute, at pH 9. The results from HPLC-RID method showed that the pH has a profound effect in the platinum-catalyzed oxidation of glucose and high conversion of D-glucose with the highest selectivity to D-gluconic acid was performed with 1%Pt/SBA-15 catalyst.

2010-01-01T00:00:00Z