Biochip for separating fetal cells from maternal circulation

DSpace/Manakin Repository

Biochip for separating fetal cells from maternal circulation

Show simple item record


dc.contributor.author Mohamed H. vi
dc.contributor.author Turner J.N. vi
dc.contributor.author Caggana M. vi
dc.date.accessioned 2011-06-10T10:18:30Z
dc.date.available 2011-06-10T10:18:30Z
dc.date.issued 2007 vi
dc.identifier.citation Volume 1162, Issue 2 SPEC. ISS., Page 187-192 vi
dc.identifier.issn vi
dc.identifier.uri http://tainguyenso.vnu.edu.vn/jspui/handle/123456789/13889
dc.description.abstract Isolation of fetal cells from maternal circulation is the subject of intense research to eliminate the need for currently used invasive prenatal diagnosis procedures. Fetal cells can be isolated using magnetic-activated cell sorting or fluorescence-activated cell sorting, however no technique to specifically isolate and use fetal cells for genetic diagnosis has reached routine clinical practice. This paper demonstrates the use of a micromachined device to separate fetal cells from maternal circulation based on differences in size and deformation characteristics. Nucleated fetal red blood cells range in diameter from 9 to 12 μm can deform and pass through a channel as small as 2.5 μm wide and 5 μm deep. Although the white blood cells range in diameter from 10 to 20 μm, they cannot deform and are retained by the 2.5 μm wide and 5 μm deep channels under our experimental conditions. Fetal cells were isolated from cord blood and DNA analysis confirmed their fetal origin with ruled out maternal contamination. © 2007 Elsevier B.V. All rights reserved. vi
dc.publisher Journal of Chromatography A vi
dc.subject Biochip vi
dc.subject Cell sorting vi
dc.subject Prenatal diagnosis vi
dc.subject Size separation vi
dc.title Biochip for separating fetal cells from maternal circulation vi
dc.type Article vi

Files in this item

Files Size Format View
MAGNETIC45.pdf 41.41Kb PDF View/Open

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account