Effecting of medium composition on biomass and ginsenoside production in cell suspension culture of Panax vietnamensis Ha et Grushv.

Abstract

We established cell suspension culture on Panax vietnamensis and some attempts have been made to increase ginsenoside yield of ginseng cell culture through manipulation various culture factors and process variable. Half and full strength MS medium were found to be equally suitable for both biomass as well as ginsenoside production. The biomass production and ginsenoside yield were obtained 9.8 g/L DW and 6.81 mg/g DW, respectively. The effect of initial sucrose concentrations were also investigated in suspension cultures of P. vietnamensis for biomass and production of ginseng saponin (secondary metabolite). The final dry cell weight was increased from 5.4 to 10.3 g/L with an increase of initial sucrose concentration from 20 to 50 g/L, but an even higher sucrose concentration of 60 g/L seemed to repress the cell growth, further increase of sucrose concentration upto 70 g/L led to a decrease in ginsenoside accumulation and biomass production. The maximum growth and ginsenoside production was obtained for nitrogen concentration of 30 mM.