D-xylulose reductase Expressed sequence tag (EST) Genomic DNA Oxidoreductase Xylitol dehydrogenase Xylose metabolism
Issue Date:
2004
Publisher:
Journal of Bioscience and Bioengineering
Citation:
Volume 97, Issue 6, Page 419-422
Abstract:
XdhA, which encodes a xylitol dehydrogenase gene, was cloned from Aspergillus oryzae genomic DNA. It consists of 1214 bp structural region, which is interrupted by two introns, and encodes 358-amino-acid protein (38,197 Da). It is similar to the known NAD+-dependent xylitol deliydrogenase (EC 1.1.1.9). The gene was expressed in Escherichia coli BL21-AI using a T7 promoter. The cell-free extract of the transformant showed a 36.5 kDa band upon SDS-PAGE and NAD+-depeadent xylitol dehydrogenase activity.
