In this paper, somatic embryogenesis induction of Eustoma grandiflorum is presented. Each in vitro-derived leaf was horizontally cut into two segments and cultured on MS (Murashige and Skoog, 1962) medium supplemented with BA, NAA or 2,4-D, individually or in combination. Embryogenic calli formation was observed on the leaf segments cultured on MS medium supplemented with various concentrations of 2,4-D (0.5, 1.0, 2.0, 3.0 or 4.0 mg l-1) in the dark. Embryonic callus formation rate was 85.5% in all leaf segments on MS medium supplemented with 0.5 mg l-1 2,4-D after 30 days of culture. Embryogenic calli were subcultured on MS medium supplemented with 1.0 mg l -1 NAA and BA (0.5, 1.0, 2.0, 3.0, 5.0 mg l-1) to induce embryo-like structures (ELSs or globular embryos). ELSs were obtained at a high frequency from embryogenic calli cultured on MS medium supplemented with 0.5 mg l-1 BA and 1.0 mg l-1 NAA after 15 days of culture. Somatic embryo formation occurred on MS medium supplemented with BA at various concentrations (0.5, 1.0, 2.0, 3.0, 4.0 or 5.0 mg l-1) and 100% somatic embryo-derived plantlets were recovered after the conversion of mature embryos on MS medium containing 1.0 mg l-1 BA. Normal development was observed after 21 days of culture.
