DSpace
 

Tai Nguyen So - Vietnam National University, Ha Noi - VNU >
TRƯỜNG ĐẠI HỌC CÔNG NGHỆ >
PTN Micro Nano >
Articles of Universities of Vietnam from Scopus >

Search

Please use this identifier to cite or link to this item: http://tainguyenso.vnu.edu.vn/jspui/handle/123456789/12696

Title: Somatic embryogenesis through pseudo-bulblet transverse thin cell layer of Lilium longiflorum
Authors: Nhut D.T.
Van Le B.
Minh N.T.
De Silva J.T.
Fukai S.
Tanaka M.
Tran Thanh Van K.
Keywords: Globular embryo
Lilium longiflorum
Pseudo-bulblet
Regeneration
Sucrose
Thin cell layer
Issue Date: 2002
Publisher: Plant Growth Regulation
Citation: Volume 37, Issue 2, Page 193-198
Abstract: Somatic embryogenesis was achieved directly from pseudo-bulblet transverse thin cell layers (tTCL) of Lilium longiflorum. Embryo-like structures (globular embryos) were obtained from different size explants of pseudo-bulblet tTCLs after 45 days culture on Murashige and Skoog, 1962 (MS) medium containing 5.4 μM naphthalene acetic acid (NAA) and 1.1 μM thidiazuron (TDZ). The embryo-like structures were then isolated and mass proliferated on MS medium, containing 5.4 μM NAA and 0.4 μM TDZ, every 45 days. A 0.8-1.0 mm thick explant was shown to be optimal for obtaining the highest number of embryo-like structures. For plant regeneration these structures were transferred to hormone-free MS medium with 30 g/l sucrose. All of these structures formed plantlets after 90 days culture.
URI: http://tainguyenso.vnu.edu.vn/jspui/handle/123456789/12696
ISSN: 1676903
Appears in Collections:Articles of Universities of Vietnam from Scopus

Files in This Item:

File SizeFormat
HCM_U457.pdf47.31 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback