Somatic embryogenesis was achieved directly from pseudo-bulblet transverse thin cell layers (tTCL) of Lilium longiflorum. Embryo-like structures (globular embryos) were obtained from different size explants of pseudo-bulblet tTCLs after 45 days culture on Murashige and Skoog, 1962 (MS) medium containing 5.4 μM naphthalene acetic acid (NAA) and 1.1 μM thidiazuron (TDZ). The embryo-like structures were then isolated and mass proliferated on MS medium, containing 5.4 μM NAA and 0.4 μM TDZ, every 45 days. A 0.8-1.0 mm thick explant was shown to be optimal for obtaining the highest number of embryo-like structures. For plant regeneration these structures were transferred to hormone-free MS medium with 30 g/l sucrose. All of these structures formed plantlets after 90 days culture.