From the methanol extract of Dracaena cambodiana roots two unusual 22S-spirostane steroids (1β,3β,14α,20R,22S,25R)-spirost-5-ene-1, 3,14-triol (1) and (1β,3β,14α,15α,20R,22S,25R)-spirost-5- ene-tetrol (2) have been isolated, together with a known 22R-spirostane compound, namogenin B (3). Their structures were elucidated by spectroscopic and spectrometric methods, including HRMS and extensive 1D and 2D NMR spectroscopy. Compound 1 showed significant antimicrobial activity against Pseudomonas aeruginosa, Staphylococcus aureus and Fusarium oxysporum, with MIC values of 45.0, 25.0 and 50.0 μg/mL, respectively.
