Impact parameters on hybridization process in detecting influenza virus (type A) using conductimetric-based DNA sensor

Abstract

This paper report various impact parameters on hybridization of probe/target DNA to detect the influenza virus (type A-H5N1) such as hybridization temperature, probe concentration, mismatch target and hybridization time. The DNA probe was attached to sensor surface by means of covalent bonding between amine of 3-aminopropyl-triethoxy-silance (APTS) and phosphate group of DNA sequence. The hybridization of probe/target DNA strands were detected by changing the surface conductance of sensors, which leads to the change in output signal of the system. The results reveal that the DNA sensor can detect as low as 0.5 nM of target DNA in real samples. The response time of DNA sensor is approximately 4 min, and the sensitivity of DNA sensor is about 0.03 mV/nM. © 2009 Elsevier B.V. All rights reserved.