We studied to prepare substrates for Surface-Enhance Raman Scattering (SERS). Gold
nanoparticles were produced by laser ablation of gold plate in ethanol. The average size of gold nanoparticles is 13nm. The gold nanoparticle colloid was allowed to dry on a silicon wafer to
prepare SERS substrate. Using the gold nanoparticle substrates we could obtain SERS spectrum of Rhodamine 6G molecules adsorbed on gold nanoparticles. The Raman signal was enhanced
strongly by our SERS substrate. This result demonstrates that the metal nanoparticles synthesized by laser ablation in clean liquid can be used to prepare SERS substrate for molecular detection in our laboratory.