To enhance monitoring capacity for pesticide residues in agricultural produce and environmental
samples in Vietnam, a simple and rapid immunoassay for cyclodienes was developed. The assay showed a
good sensitivity for endosulfan with an IC50 of 1-2?g/L. A wide cross reactivity for its metabolites and other
cyclodienes such as aldrin, endrin, dieldrin, heptachlor led to formatting an ELISA (Enzyme-Linked
ImmunoSorbent Assay) kit with a broad specificity for cyclodienes. A simple sample preparation protocol
also was developed for diverse samples including water, soil, vegetables and fruits. During the years 2002-
2003 the cyclodiene ELISA kits were supplied to various research institutions in South and North Vietnam
to collaboratively monitor the pesticide residues in water, soil, vegetable and fruit in the North, South and
Central Vietnam. A total of 450 samples of vegetable and grapes were screened for cyclodiene residues
using the ELISA. The validation of 10% of positive samples by gas chromatography (GC) method
confirmed that the results of ELISA correlated well and were reliable. ?? 2007 American Chemical Society.
