The objective of the present study was to develop an experimentally obese mice model
and to investigate antiobesity and body weight reducing effect of Fortunella japonica peel extract
fractions.
Malemice (Mus musculus, Swiss raceweighed at 14-15g) purchased fromNational Institute of Hygiene
and Epidemiology (NIHE) were divided into 5 groups in which, the first group was fed with normal pellet
diet (NPD) (Standard chow from NIHE), and other groups (2-5 groups) were fed high fat diet (HFD)
containing 58% calories as fat for a period of 15 days.
The HFD mice exhibited significant increase in body weight about 44% as compared to NPD
group at that time (p< 0.05). Besides, the HFD mice showed significant increase in blood total
cholesterol, blood triglyceride LDL and glucose concentrations to be 32.20%, 44.0%, 57.50% and
24.60% respectively as compared to NPD mice. In addition, the effects of daily repeated oral
administration for 8 days of 800 mg/kg of ethylacetate, chloroform and ethanol extract fractions
from Fortunella japonica peels were studied. The obtained results showed that the body weights
of HFD mice treated by different extract fractions were reduced by 9.2% (for ethylacetate extract),
8.2% (for choloroform extract) and 4.7% (for ethanol extract) as compared to vehicle (control,
obese mice were treated by water replaced the extracts (p<0.05).
Besides, HFD mice treated by different extract fractions exhibited significant decrease in
blood total cholesterol to 13.2% (for ethanol extract), 1.4% (for chloroform extract), 0.7% (for
ethylacetate), in triglycerid 13% (for ethanol extract), 15.2% (for chloroform extract), 16.2% (for
ethylacetate extract), in glucose 0.9% (for ethanol extract), 23% (for chloroform), 18.5% (for
ethylacetate extract) as compared to control (p<0.05).
