Cell suspension culture Panax ginseng C. A. Meyer: Role of plant growth regulators and medium composition on biomass and ginsenoside production

Abstract

We established cell suspension culture on ginseng and some attempts have been made to increase ginsenoside yield of ginseng cell culture through manipulation various culture factors and process variable. The maximum biomass yields of cell suspension culture of ginseng was obtained in medium containing 2,4-D as compared to IBA or NAA. However, ginsenoside production was much higher in IBA or NAA containing medium and 7 mg/L IBA was determined to be optimal for cell growth (10.1 mg/L DW) and ginsenoside production (7.2 mg/g DW). Addition of cytokinin (BA and kinetin) did not affect cell growth but ginsenoside production was increased when the medium supplemented with 0.5 mg/L BA or 0.5 mg/L kinetin. Half and full strength MS medium were found to be equally suitable for both biomass as well as ginsenoside production. At various sucrose concentration investigated, 30 g/L sucrose enhanced biomass yield as well as ginsenoside production and further increase of sucrose concentration upto 70 g/L led to a decrease in ginsenoside accumulation and biomass production. The maximum growth and ginsenoside production was obtained for nitrogen concentration of 30 mM