Vector construction and transformation of 4CL1 gene into Chinaberrytree (Melia azedarach L.)

Abstract

The gene 4CL1 was isolated from Chinese red pine (Pinus massoniana Lamb) and ligated into vector pPTN289 to perform transformation vector pPTN289-4CL1. This construction was transformed into Agrobacterium tumefaciens strain C58, and then transformed into Chinaberrytree (Melia azedarach L.). The transgenic Chinaberrytree was screened on selection medium (MS + 0.5mg/l 6-BA + 1mg/l vitamine B5 + 30g/l sucrose + 8g/l agar + 500mg/l Cefotaxime + 1mg/l PPT) and then extracted total DNA and tested the existence of interested gene using PCR method. The result shows that two tested Chinaberrytree samples are positive with 4CL1 gene as same as the positive control, while the nagative control (wild Chinaberrytree) is nagative with 4CL1 gene.